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Identification, Purification, and Characterization of Staphylococcal Superantigens
Joseph A Merriman1, Patrick M Schlievert2
1Department of Microbiology, University of Iowa, 51 Newton Road, Bowen Science Building 3-403, Iowa City, IA, 52242, USA.
Abstract:
Purifying natively produced staphylococcal superantigens is an important process in the study of these proteins, as many common methods of protein purification are affected by staphylococcal protein A contamination. Here, we describe a proven approach for identifying superantigens in vitro as well as for purifying novel superantigens both in His-tagged and native forms using modern genetic tools coupled with thin-layer isoelectric focusing.
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