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Induction of Human Regulatory T Cells with Bacterial Superantigens

Stefano Caserta1, Amanda L Taylor2, Nadia Terrazzini3

  • 1Division of Clinical Medicine, BSMS-Brighton and Sussex Medical School, University of Sussex, Medical Research Building, Falmer, BN1 9PS, UK. s.caserta@bsms.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|December 18, 2015
PubMed

Regulatory T cells (Tregs) that suppress the activation of immune effector cells limit immunopathology and are fast emerging as therapeutic targets for autoimmune and cancer disease. Tools enabling Treg in vitro-induction, expansion, and characterization and manipulation will help future clinical developments. In this chapter, we describe in detail how to use bacterial superantigens to induce human Tregs efficiently from peripheral blood mononuclear cells. How to assess human Treg phenotype and suppressive capacity are also described. Technical details, variations, and alternative experimental conditions are provided.

Keywords:
Cell purificationHumanImmunological methodsPBMCRegulatory T cellsSuperantigenSuperantigen stimulationSuppression assayTreg phenotype

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