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Updated: Mar 28, 2026

Whole-Kidney Three-Dimensional Staining with CUBIC

Whole-Kidney Three-Dimensional Staining with CUBIC

Published on: July 18, 2022

Biopsychronology: A Method Using Live Tissue Staining to Image Cell Function in the Kidney.

Muhammad Imtiaz Ashraf¹, Dietmar Fries², Werner Streif³

¹Daniel Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Center of Operative Medicine, Medical University Innsbruck, Innsbruck, Austria.

Methods in Molecular Biology (Clifton, N.J.)

|December 18, 2015

Summary

This study introduces a rapid method using fine needle biopsies and live cell staining to assess organ graft health before transplantation, potentially preventing complications and improving outcomes.

Keywords:

Biopsy Kidney Real-time live confocal microscopy

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Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging

Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging

Published on: March 28, 2020

Area of Science:

Biomedical Engineering
Transplant Surgery
Cellular Biology

Background:

Graft viability assessment is crucial to avoid transplant failure and optimize patient outcomes.
Current methods for pre-transplant graft evaluation are limited, leading to potential complications like delayed graft function.
Minimizing unnecessary interventions and improving graft survival are key goals in transplantation.

Purpose of the Study:

To develop and validate a rapid, minimally invasive method for assessing organ graft integrity before transplantation.
To provide real-time functional and structural information on organ viability.
To reduce the risk of graft loss and improve post-transplant management.

Main Methods:

Utilizing fine needle biopsies for sample acquisition.
Employing specific dyes to stain living cells, focusing on mitochondrial status and cellular integrity.
Implementing live confocal microscopy for real-time analysis of stained cells.

Main Results:

The developed method provides organ functional and structural intactness information within minutes.
A pilot study using rodent kidneys confirmed the method's suitability for detecting organ damage from ischemia or reperfusion.
The procedure demonstrated minimal sample preparation and data acquisition time.

Conclusions:

This novel approach offers a rapid and effective means to monitor organ graft status pre-transplantation.
The method has the potential to significantly improve clinical outcomes by enabling informed decisions regarding graft suitability.
It is suitable for detecting damage from unphysiological stress, aiding in the selection of viable organs for transplantation.