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Updated: Mar 28, 2026

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

A novel method for endothelial cell isolation.

Qiqi Mao¹, Xianing Huang¹, Jian He¹

¹National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

Oncology Reports

|December 18, 2015

Summary

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A new method efficiently purifies newborn endothelial cells (CD105+) from tumor tissues using collagenase digestion with vortexing. This technique yields high-purity cells with typical endothelial functions, ideal for studying vessel-based diseases.

Area of Science:

Cell Biology
Oncology
Biotechnology

Background:

Tumor angiogenesis requires precise understanding of endothelial cell behavior.
Efficient isolation of pure endothelial cells from tumor microenvironments is challenging.
CD105 is a well-established marker for endothelial progenitor cells.

Purpose of the Study:

To develop a rapid and effective method for purifying CD105+ endothelial cells from mouse tumor tissues.
To optimize cell isolation protocols for enhanced yield and purity.
To validate the functional characteristics of isolated endothelial cells for research applications.

Main Methods:

Tumor tissues from C57BL/6 mice bearing Lewis lung cancer cells were minced.
Cells were subjected to collagenase type I digestion, with and without vortexing.

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Last Updated: Mar 28, 2026

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

Isolation of Valvular Endothelial Cells

Isolation of Valvular Endothelial Cells

Published on: December 29, 2010

Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples

Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples

Published on: February 3, 2023

CD105+ cells were isolated using Dynabeads and characterized by FACS, Dil-Ac-LDL uptake, and tube formation assays.

Main Results:

Vortex-assisted digestion significantly increased cell yield (5.70x10^4 vs 0.32x10^4 cells) and purity compared to non-vortexed digestion (P<0.01).
Isolated CD105+ cells demonstrated high purity and expressed CD105 on their surface.
Functional assays confirmed typical endothelial cell behavior, including Dil-Ac-LDL uptake and capillary-like structure formation.

Conclusions:

The modified collagenase digestion method with vortexing provides a highly efficient and pure isolation of CD105+ endothelial cells from tumors.
This optimized protocol yields functional vascular endothelial cells suitable for mechanistic studies.
The method facilitates research into the development and pathology of vessel-based diseases.