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Interleukin-17A as a Biomarker for Bovine Tuberculosis
W Ray Waters1, Mayara F Maggioli2, Mitchell V Palmer3
1National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA ray.waters@ars.usda.gov.
Clinical and Vaccine Immunology : CVI
|December 18, 2015
Summary
T helper 17 (Th17) cytokines are key in bovine tuberculosis. Interleukin-17A (IL-17A) responses correlated with reduced disease, suggesting its use as a biomarker for infection and vaccine protection.
Area of Science:
- Veterinary Immunology
- Tuberculosis Research
- Cytokine Biology
Background:
- T helper 17 (Th17) cytokines play a dual role in tuberculosis immunity, mediating both protective and detrimental inflammatory responses.
- Understanding the role of interleukin-17 (IL-17) in bovine tuberculosis is crucial for developing effective control strategies.
Purpose of the Study:
- To investigate the biological significance of IL-17-associated cytokines in cattle experimentally infected with Mycobacterium bovis.
- To evaluate the potential of Th17 cytokines as biomarkers for infection and vaccine-induced protection against bovine tuberculosis.
Main Methods:
- Transcriptome sequencing (RNA-Seq) was employed to analyze gene expression in peripheral blood mononuclear cells (PBMCs) from M. bovis-infected cattle.
- Quantification of Th17-associated cytokine gene expression following stimulation with purified protein derivative (PPD).
- Correlation analysis between cytokine responses, disease burden, and vaccination status.
Main Results:
- Numerous Th17-associated cytokine genes (IL-17A, IL-17F, IL-22, IL-19, IL-27) showed significant upregulation (>9-fold) in response to PPD stimulation.
- Vaccination against bovine tuberculosis elicited robust IL-17A, IL-17F, IL-22, and IL-27 responses.
- Lower IL-17A responses in vaccinated animals post-challenge correlated with reduced M. bovis disease burden.
- IL-17A and interferon gamma (IFN-γ) responses were highly correlated and demonstrated similar diagnostic potential.
Conclusions:
- Th17-associated cytokines, particularly IL-17A, are significantly involved in the immune response to Mycobacterium bovis infection in cattle.
- IL-17A serves as a potential biomarker for assessing infection status and vaccine efficacy in bovine tuberculosis.
- These findings support the utility of Th17 cytokines in monitoring and managing bovine tuberculosis.
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