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Identification and Characterization of an Insulin-Like Receptor Involved in Crustacean Reproduction.

O Sharabi1, R Manor1, S Weil1

  • 1Department of Life Sciences (O.S., R.M., S.W., E.D.A., Y.L., T.L., A.S.), The National Institute for Biotechnology in the Negev (S.W., E.D.A., A.S.), and Avram and Stella Goldstein-Goren Department of Biotechnology Engineering (I.K.), Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; GeneCology Research Centre (J.A., T.V.), Faculty of Science, Health, Education, and Engineering, University of the Sunshine Coast, Maroochydore, Queensland 4558, Australia; and Department of Earth, Environmental, and Biological Sciences (P.B.M.), Science and Engineering Faculty, Queensland University of Technology, Brisbane, Queensland 4000, Australia.

Endocrinology
|December 19, 2015
PubMed
Summary

Researchers identified the insulin-like receptor (Mr-IR) in prawns, revealing its role in regulating the androgenic gland (AG). Silencing Mr-IR advanced male traits but did not cause a sexual shift, suggesting complex regulation beyond a single receptor.

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Area of Science:

  • Crustacean Endocrinology
  • Molecular Endocrinology
  • Reproductive Biology

Background:

  • The androgenic gland (AG) insulin-like peptide (IAG) is hypothesized to regulate masculinity in crustaceans.
  • Downstream signaling pathways of IAG remain largely uncharacterized.

Purpose of the Study:

  • To identify and characterize the insulin-like receptor (Mr-IR) in the prawn Macrobrachium rosenbergii.
  • To investigate the functional role of Mr-IR in sexual differentiation and AG regulation.

Main Methods:

  • Construction of a comprehensive M. rosenbergii transcriptomic library.
  • Sequencing of the complete Mr-IR cDNA.
  • RNA interference (RNAi) to silence Mr-IR in male prawns.
  • Ligand blot assay with recombinant Mr-IAG.

Main Results:

  • Mr-IR was identified and its cDNA sequenced; expression was detected in multiple tissues, including the AG and gonads.
  • Mr-IR silencing in males advanced secondary male traits, caused AG hypertrophy, and increased Mr-IAG production.
  • A direct ligand-receptor interaction between Mr-IAG and Mr-IR was confirmed.
  • Mr-IR silencing did not induce a sexual shift, unlike Mr-IAG gene silencing.

Conclusions:

  • Mr-IR plays a regulatory role in the androgenic gland of M. rosenbergii.
  • Sexual differentiation in crustaceans involves complex signaling pathways, likely involving multiple receptors beyond Mr-IR.
  • The findings highlight the intricate mechanisms governing sexual maintenance and differentiation in decapod crustaceans.