Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.8K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.8K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

2.1K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
2.1K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

1.2K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
1.2K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

798
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
798
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

850
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
850

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First worldwide multicenter validation of the POLARIS preclinical polarizer across biological models and imaging paradigms.

Research square·2026
Same author

Development and First-in-Human Translation of Hyperpolarized [1-<sup>13</sup>C]Alpha-Ketoglutarate MR Spectroscopy in the Brain.

Sensors (Basel, Switzerland)·2026
Same author

Evaluation of treatment response in patients with recurrent grade 4 glioma using hyperpolarized [1-<sup>13</sup>C]pyruvate MRI.

Research square·2026
Same author

Spatial distribution of hyperpolarized [1-<sup>13</sup>C]pyruvate MRI and metabolic PET in the human brain.

Imaging neuroscience (Cambridge, Mass.)·2025
Same author

Correction: Chen et al. Multivariate Framework of Metabolism in Advanced Prostate Cancer Using Whole Abdominal and Pelvic Hyperpolarized 13C MRI-A Correlative Study with Clinical Outcomes. <i>Cancers</i> 2025, <i>17</i>, 2211.

Cancers·2025
Same author

Estimating Sensitivity Maps for X-Nuclei Magnetic Resonance Spectroscopic Imaging.

ArXiv·2025

Related Experiment Video

Updated: Mar 28, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

11.1K

Development and testing of hyperpolarized (13)C MR calibrationless parallel imaging.

Yesu Feng1, Jeremy W Gordon1, Peter J Shin1

  • 1Department of Radiology and Biomedical Imaging, UCSF, San Francisco, CA, USA.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|December 19, 2015
PubMed
Summary

A new method enhances hyperpolarized carbon-13 MRI scans, enabling faster imaging with high resolution for better diagnostics. This technique improves speed without calibration for advanced medical imaging applications.

Keywords:
Carbon-13HyperpolarizationParallel imaging

More Related Videos

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

7.3K
Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
09:05

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Published on: July 8, 2025

1.6K

Related Experiment Videos

Last Updated: Mar 28, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

11.1K
Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

7.3K
Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
09:05

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Published on: July 8, 2025

1.6K

Area of Science:

  • Medical Imaging
  • Magnetic Resonance Imaging
  • Nuclear Magnetic Resonance

Background:

  • Hyperpolarized (13)C MRI offers unique metabolic insights but is limited by long acquisition times.
  • Existing parallel imaging techniques often require complex calibration procedures.
  • The need for accelerated imaging is critical for clinical translation and broader applications.

Purpose of the Study:

  • To adapt and validate a calibrationless parallel imaging technique for hyperpolarized (13)C MRI.
  • To achieve high spatial resolution and large field-of-view (FOV) imaging.
  • To demonstrate the technique's efficacy in phantom and in vivo studies.

Main Methods:

  • Modification of a previously developed calibrationless parallel imaging technique for (1)H MRI.
  • Application to hyperpolarized (13)C MRI using a 2D symmetric Echo Planar Imaging (EPI) readout.
  • Implementation of random blips in the phase encode dimension for retrospective and prospective undersampling.
  • Acquisition of data in phantom and in vivo rat models.

Main Results:

  • Successful demonstration of the modified technique on hyperpolarized (13)C MRI data.
  • Achieved a 2-fold acceleration factor with minimal loss of image quality.
  • Reconstructed images showed excellent qualitative agreement compared to fully sampled data.
  • Phantom and in vivo rat studies confirmed the technique's feasibility and performance.

Conclusions:

  • The adapted calibrationless parallel imaging technique is effective for accelerated hyperpolarized (13)C MRI.
  • This method enables high-resolution, large FOV imaging crucial for clinical applications.
  • Future work can explore multi-dimensional undersampling for even greater acceleration.