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Antisense Deoxyoligonucleotides Inhibit Activities of Matrix Metalloproteinase-2 in Human Fibrosarcoma HT1080 Cells

Jung Sun Park, Dong On Yang, Seon Hee Lim

    Cancer Research and Treatment
    |December 19, 2015
    PubMed
    Abstract

    Insights

    Antisense oligodeoxynucleotides (ODNs) targeting matrix metalloproteinase-2 (MMP-2) effectively inhibited tumor cell invasion. Antisense-6 demonstrated significant dose-dependent reduction in MMP-2 activity, mRNA, protein, and cell invasion, suggesting therapeutic potential.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Oncology

    Background:

    • Matrix metalloproteinase-2 (MMP-2), a 72 kDa type IV collagenase, is crucial for tumor cell migration and growth.
    • MMP-2 facilitates tumor invasion by degrading the basement membrane.
    • MMP-2 activation correlates with the invasiveness of various cancers.

    Purpose of the Study:

    • To investigate sequence-specific phosphorothioated oligodeoxynucleotides (ODNs) for inhibiting MMP-2 translation.
    • To evaluate the impact of these ODNs on tumor cell invasiveness.

    Main Methods:

    • Eight types of antisense ODNs were designed and transfected into HT1080 fibrosarcoma cells.
    • MMP expression was assessed using gelatin zymography, Western blot, and Northern blot.
    • In vitro invasion was evaluated using matrigel assays.

    Main Results:

    • Antisense-5, antisense-6, and antisense-7 significantly reduced MMP-2 activity in HT1080 cell culture supernatant.
    • Antisense-6 demonstrated a dose-dependent inhibition of MMP-2 mRNA and protein expression.
    • Antisense-6 treatment resulted in a dose-dependent reduction of in vitro tumor cell invasion.

    Conclusions:

    • Antisense-6 shows promise as a therapeutic agent for inhibiting tumor invasion and metastasis.
    • Targeting MMP-2 translation with specific ODNs is a viable strategy for cancer therapy.
    Keywords:
    Antisense oligodeoxynucleotidesMatrix metalloproteinases-2MetastasisTumor invasion

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