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Adiponectin Isoforms and Leptin Impact on Rheumatoid Adipose Mesenchymal Stem Cells Function
1Department of Immunology and Pathophysiology, Institute of Rheumatology, Ul. Spartańska 1, 02-637 Warsaw, Poland.
Stem Cells International
|December 19, 2015
Summary
High/middle molecular weight adiponectin isoforms stimulate rheumatoid arthritis adipose stem cells
Area of Science:
- Immunology
- Cell Biology
- Rheumatology
Background:
- Adiponectin and leptin are implicated in rheumatoid arthritis (RA) pathogenesis.
- Adipose-derived stem cells (ASCs) possess immunomodulatory functions relevant to RA.
Purpose of the Study:
- To investigate the impact of adiponectin and leptin on the immunomodulatory functions of ASCs from RA patients.
- To assess how these adipokines affect ASCs' interactions with rheumatoid synovial fibroblasts (RA-FLS) and peripheral blood mononuclear cells (PBMCs).
Main Methods:
- ASCs from RA patients were isolated and stimulated with leptin and different adiponectin isoforms (LMW, HMW/MMW).
- Secretory activity of ASCs and their effects on RA-FLS proliferation and PBMC responses (proliferation, cytokine secretion) were analyzed.
Main Results:
- HMW/MMW adiponectin significantly upregulated the secretion of TGFβ, IL-6, IL-1Ra, PGE2, IL-8, and VEGF by RA-ASCs.
- HMW/MMW adiponectin partially reduced the proproliferative effect of ASCs on RA-FLS but did not alter IL-6 secretion in FLS.
- HMW/MMW adiponectin-treated ASCs retained anti-inflammatory properties, inhibiting PBMC proliferation and inducing IL-10 secretion.
Conclusions:
- HMW/MMW adiponectin enhances the secretory potential of rheumatoid ASCs.
- The impact of HMW/MMW adiponectin on ASCs' functional effects on RA-FLS and PBMCs is moderate, suggesting a complex role in RA pathogenesis.
