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Updated: Mar 28, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae
Published on: November 8, 2006
Complementary roles of the Cek1 and Cek2 MAP kinases in Candida albicans cell-wall biogenesis
Inês Correia1, Elvira Román1, Daniel Prieto1
1Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza de Ramón y Cajal s/n, E-28040 Madrid, Spain.
Aims:
To investigate whether Cek2 (as Cek1) is involved in the biogenesis of the fungal cell wall and to uncover differences and similitudes between both proteins.
Materials & Methods:
We used molecular genetics to characterize the role of Cek2 in MTL-heterozygous cells.
Results:
Deletion of CEK2 (similar to CEK1) renders cells sensitive to cell-wall-interfering drugs and, when overexpressed, Cek2 can become phosphorylated upon the same stimuli that activate Cek1. This is dependent on elements of the sterile-vegetative growth (SVG) pathway. Cek2, contrary to Cek1, mediates a transcriptional activity in a C. albicans-adapted two-hybrid system that is essential for Cek1-Cek2 interaction.
Conclusion:
Cek2 has a cryptic role in cell-wall biogenesis and its role is not entirely redundant to Cek1.
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