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Related Experiment Videos

Transepithelial prostacyclin gradient in isolated canine trachea.

D H Eidelman1, W S Powell, S Bellofiore

  • 1Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|July 1, 1989
PubMed
Summary

Airway epithelium acts as a barrier to prostacyclin (PGI2) movement. This bronchodilator, produced in the airway submucosa, shows limited relaxation effects when applied to the mucosal side, unlike the serosal side.

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Area of Science:

  • Respiratory Physiology
  • Pulmonary Pharmacology

Background:

  • Cyclooxygenase (COX) products of arachidonic acid modulate airway smooth muscle.
  • Prostacyclin (PGI2) is a significant bronchodilator synthesized by airway tissues.
  • Previous studies identified COX products in bronchoalveolar lavage from canine lungs.

Purpose of the Study:

  • To investigate if canine airway epithelium impedes prostacyclin (PGI2) movement.
  • To quantify PGI2 metabolite (6-oxo-PGF1 alpha) concentrations across canine tracheal segments.
  • To determine the role of airway epithelium versus submucosa in PGI2 production and function.

Main Methods:

  • Isolated canine tracheal segments (CTS) were mounted in Ussing chambers.
  • 6-oxo-PGF1 alpha concentrations were measured on mucosal and serosal sides using radioimmunoassay and HPLC.

Related Experiment Videos

  • PGI2 production was assessed using [3H]arachidonic acid and calcium ionophore A23187.
  • Tracheal relaxation responses to PGI2 were measured on mucosal and serosal applications.

    • Main Results:

    • 6-oxo-PGF1 alpha concentrations were significantly higher on the serosal side compared to the mucosal side of CTS.
    • A strong correlation existed between 6-oxo-PGF1 alpha levels measured on both sides of the tracheal segments.
    • PGI2 was the predominant COX product in the submucosa, with minimal PGI2 production by the mucosa.
    • PGI2 induced significant relaxation only when applied to the serosal side of precontracted tracheal segments.

    Conclusions:

    • The canine airway epithelium acts as a barrier to prostacyclin (PGI2) movement.
    • PGI2 production primarily occurs in the airway submucosa, not the epithelium.
    • The functional effects of PGI2 on airway smooth muscle are dependent on its site of application, with serosal application being more effective.