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Specific alterations in transcript prevalence during the yeast life span.

N K Egilmez1, J B Chen, S M Jazwinski

  • 1Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70112.

The Journal of Biological Chemistry
|August 25, 1989
PubMed
Summary
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Aging yeast cells show changes in gene expression. Researchers identified specific genes that are more active in young or old budding yeast (Saccharomyces cerevisiae), indicating altered transcript levels during aging.

Area of Science:

  • Cellular biology
  • Molecular genetics
  • Aging research

Background:

  • The budding yeast Saccharomyces cerevisiae has a finite lifespan, with cells undergoing age-related changes.
  • Understanding molecular alterations during aging is crucial for cellular senescence studies.

Purpose of the Study:

  • To investigate age-associated changes in messenger RNA (mRNA) prevalence in yeast.
  • To identify specific yeast genes with differential expression patterns throughout the cellular lifespan.

Main Methods:

  • Differential hybridization screening using a yeast genomic DNA library.
  • Probing with complementary DNA (cDNA) from young and old yeast polyadenylated (poly(A)+) mRNA.
  • Genomic blot and RNA dot blot analyses to confirm gene expression patterns.

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Main Results:

  • Six distinct yeast genes exhibiting differential expression during aging were isolated.
  • Five genes were classified as young-specific, and one gene was classified as old-specific.
  • Expression of selected young- and old-specific genes was independent of the cell cycle stage.

Conclusions:

  • Aging in Saccharomyces cerevisiae is associated with specific alterations in the abundance of certain gene transcripts.
  • These findings suggest a molecular basis for age-related changes in yeast cellular function.