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Correlation between apolipoprotein A-IV and triglyceride concentrations in human sera.
L Lagrost1, P Gambert, S Meunier
1Laboratoire de Biochimie Médicale, Faculté de Médecine, Dijon, France.
Journal of Lipid Research
|May 1, 1989
Summary
Apolipoprotein A-IV levels increase with higher triglyceride concentrations in fasting individuals. This suggests very low-density lipoprotein secretion may influence plasma apolipoprotein A-IV levels.
Area of Science:
- Lipid Metabolism
- Clinical Chemistry
- Biochemistry
Background:
- Apolipoprotein A-IV (ApoA-IV) is a protein found in plasma.
- Its relationship with lipid levels, particularly triglycerides, requires further elucidation.
Purpose of the Study:
- To investigate the correlation between serum apolipoprotein A-IV concentration and triglyceride levels in fasted humans.
- To explore the potential contribution of very low-density lipoprotein (VLDL) secretion to plasma ApoA-IV levels.
Main Methods:
- A competitive enzyme immunoassay was developed to measure ApoA-IV concentration.
- Sera from 105 fasted human subjects with varying triglyceride levels (20-474 mg/dl) were analyzed.
- Subjects with detectable chylomicrons and total cholesterol >260 mg/dl were excluded.
Main Results:
- Apolipoprotein A-IV concentration significantly increased with rising triglyceride levels (P < 0.001).
- A significant positive linear correlation (r = 0.61, P < 0.001) was observed between serum ApoA-IV and triglyceride concentrations.
- ApoA-IV was not detected in the triglyceride-rich lipoprotein fraction post-gel filtration.
Conclusions:
- Serum apolipoprotein A-IV levels are positively associated with triglyceride concentrations in fasted individuals.
- VLDL secretion is a potential contributor to plasma ApoA-IV levels in the fasting state.
- Further research is needed to fully understand the regulatory mechanisms of ApoA-IV.