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Naked-eye quantitative aptamer-based assay on paper device.

Yun Zhang1, Dong Gao1, Jinlong Fan1

  • 1Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, PR China.

Biosensors & Bioelectronics
|December 20, 2015
PubMed
Summary

Researchers developed low-cost, naked-eye aptamer assays using microfluidic paper devices. These novel methods enable simple, portable, and cost-efficient point-of-care testing without electronic readers.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Materials Science

Background:

  • Aptamer-based assays offer high specificity for analyte detection.
  • Microfluidic paper-based analytical devices (μPADs) provide a low-cost platform for diagnostics.
  • Existing μPAD methods often require external readers, limiting their accessibility.

Purpose of the Study:

  • To design novel, equipment-free quantitative detection methods for aptamer-based assays on μPADs.
  • To enable naked-eye readout for improved portability and cost-efficiency.
  • To demonstrate the utility of these methods for point-of-care applications.

Main Methods:

  • Development of two new detection motifs for quantitative μPAD measurements.
  • A length-measuring method based on iodine-starch complex formation for color change.
  • A counting method utilizing an oxidation-reduction reaction for detection.

Main Results:

  • Successful demonstration of sensitive and specific detection of adenosine.
  • Quantitative measurements achieved using naked-eye visual assessment.
  • Assays performed effectively in buffer samples and undiluted human serum.

Conclusions:

  • The proposed naked-eye quantitative aptamer assays on μPADs are simple, cost-efficient, and user-friendly.
  • These methods hold significant potential for point-of-care applications, especially in resource-constrained settings.
  • The developed motifs offer a viable alternative to electronic readers for μPAD-based diagnostics.