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Characterization and Immunogenicity of Outer Membrane Vesicles from Brucella abortus
Gagandeep Kaur1, Satparkash Singh1, B V Sunil Kumar1
1a School of Animal Biotechnology , Guru Angad Dev Veterinary and Animal Sciences University , Ludhiana , India.
Abstract:
Bovine brucellosis is a worldwide spread zoonotic disease. The objectives of this study were characterization of outer membrane vesicles from B. abortus and to evaluate their immunogenicity in mice. For this purpose, OMVs were derived from B. abortus strain 99 using ultracentrifugation method. Isolated OMVs were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transmission electron microscopy which revealed spherical 20-300 nm structures rich in proteins. OMVs also showed immuno-reactivity with mice antisera in Western blot. Further, indirect ELISA showed specific and high-titer immune responses against the antigens present in OMVs suggesting their potential for a safe acellular vaccine candidate.
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