Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

3.4K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
3.4K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

5.2K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
5.2K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

19.4K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
19.4K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

9.9K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.9K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.4K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.4K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

10.1K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
10.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The prenylated pUS2 protein of pseudorabies virus contributes to phosphorylation of connexin 43 and suppression of gap junctional intercellular communication.

Journal of virology·2026
Same author

TEAD1 condensates are transcriptionally inactive storage sites on the pericentromeric heterochromatin in cancer cells.

Nature cell biology·2026
Same author

It started off as a Cys, how did it end up like this? Identifying the extent of unmodelled oxidatively modified cysteines within the Protein Data Bank.

Acta crystallographica. Section D, Structural biology·2026
Same author

In situ architecture of plasmodesmata in Physcomitrium patens resolved by cryo-electron tomography.

Nature plants·2026
Same author

Regional and Temporal Patterns of Long-Term Pseudorabies Virus Detection and Neuropathology in the Murine CNS.

Pathogens (Basel, Switzerland)·2026
Same author

The MHV-68 nuclear egress complex supports C-capsid selective capsid egress.

Journal of virology·2026

Related Experiment Video

Updated: Mar 28, 2026

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
07:10

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

6.1K

Structural Basis of Vesicle Formation at the Inner Nuclear Membrane.

Christoph Hagen1, Kyle C Dent2, Tzviya Zeev-Ben-Mordehai1

  • 1Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK.

Cell
|December 22, 2015
PubMed
Summary
This summary is machine-generated.

Researchers visualized the nuclear egress complex (NEC) forming vesicles for herpesvirus transport. This reveals the structural basis for enveloped viral capsid budding and scission at the inner nuclear membrane.

More Related Videos

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells
10:10

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells

Published on: July 3, 2025

1.2K
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
15:39

Studying Organelle Dynamics in B Cells During Immune Synapse Formation

Published on: June 1, 2019

9.7K

Related Experiment Videos

Last Updated: Mar 28, 2026

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
07:10

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

6.1K
Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells
10:10

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells

Published on: July 3, 2025

1.2K
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
15:39

Studying Organelle Dynamics in B Cells During Immune Synapse Formation

Published on: June 1, 2019

9.7K

Area of Science:

  • Cell Biology
  • Structural Biology
  • Virology

Background:

  • Vesicular transport across the nuclear envelope is a key mechanism for moving large molecules.
  • The nuclear egress complex (NEC) mediates herpesvirus capsid envelopment for nuclear export.

Purpose of the Study:

  • To elucidate the structural mechanisms of NEC-mediated vesicle formation during herpesvirus nuclear egress.
  • To understand the molecular architecture of the NEC and its role in vesicle budding and scission.

Main Methods:

  • Multi-modal imaging techniques, including in situ visualization of the NEC.
  • Cellular electron cryo-tomography to resolve lattice structures.
  • Integrative modeling with small-angle X-ray scattering data.

Main Results:

  • Visualized the NEC forming coated vesicles of specific sizes at the inner nuclear membrane.
  • Identified two distinct hexagonal lattices within the NEC protein layer.
  • Determined the molecular arrangement of the NEC coat.

Conclusions:

  • The NEC's molecular structure dictates the mechanism for budding and scission of vesicles at the inner nuclear membrane.
  • Provides structural insights into nucleo-cytoplasmic transport of large viral cargoes.