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A biologically inspired variable-pH strategy for enhancing short-chain fatty acids (SCFAs) accumulation in maize
Yao Meng1, Jan Mumme2, Heng Xu1
1State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, PR China.
Abstract:
This study investigates the feasibility of varying the pH to enhance the accumulation of short-chain fatty acids (SCFAs) in the in vitro fermentation of maize straw. The corresponding hydrolysis rate and the net SCFA yield increased as inoculum ratio (VSinoculum/VSsubstrate) increased from 0.09 to 0.79. The pH were maintained at 5.3, 5.8, 6.3, 6.8, 7.3, and 7.8, respectively. A neutral pH of approximately 6.8 was optimal for hydrolysis. The net SCFA yield decreased by 34.9% for a pH of less than 5.8, but remained constant at approximately 721±5mg/gvs for a pH between 5.8 and 7.8. In addition, results were obtained for variable and constant pH levels at initial substrate concentrations of 10, 30 and 50g/L. A variable pH increased the net SCFA yield by 23.6%, 29.0%, and 36.6% for concentrations of 10, 30 and 50g/L. Therefore, a variable pH enhanced SCFA accumulation in maize straw fermentation.
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