Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

16.1K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
16.1K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

17.6K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.6K
Homologous Recombination02:31

Homologous Recombination

65.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
65.4K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.4K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alterations in chromatin organization promote totipotent-like features in a DPPA2/DUX-dependent manner.

The EMBO journal·2026
Same author

The association of frailty with age and lifespan in mice differs by strain and sex.

The journals of gerontology. Series A, Biological sciences and medical sciences·2026
Same author

DPY30 Sticks a Fork in PDAC Immunity.

Cancer research·2026
Same author

Turning back time: a comprehensive list of interventions that decrease next-generation epigenetic aging clocks in humans.

Frontiers in genetics·2026
Same author

G quadruplex DNA facilitates a pervasive path to homologous recombination.

bioRxiv : the preprint server for biology·2026
Same author

Corrigendum to "Oculomics and AI: The eye as a biomarker for health span" [Asia-Pac J Ophthalmol 15 (1) (2026) 100282].

Asia-Pacific journal of ophthalmology (Philadelphia, Pa.)·2026

Related Experiment Video

Updated: Mar 28, 2026

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos
07:46

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

Published on: June 23, 2023

3.4K

Controlled DNA double-strand break induction in mice reveals post-damage transcriptome stability.

Jeongkyu Kim1, David Sturgill1, Andy D Tran1

  • 1Laboratory for Receptor Biology and Gene Expression, National Cancer Institute, 41 Library Drive, Bethesda, MD 20892, USA.

Nucleic Acids Research
|December 22, 2015
PubMed
Summary

DNA double-strand breaks (DSBs) can alter gene expression, but primary cells can maintain transcriptome integrity. DSB repair reverses expression changes, independent of cell cycle, suggesting limited cell-autonomous dysfunction.

More Related Videos

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

2.9K
Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
09:07

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

Published on: September 20, 2021

3.2K

Related Experiment Videos

Last Updated: Mar 28, 2026

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos
07:46

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

Published on: June 23, 2023

3.4K
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

2.9K
Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
09:07

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

Published on: September 20, 2021

3.2K

Area of Science:

  • Genetics
  • Epigenetics
  • Molecular Biology

Background:

  • DNA double-strand breaks (DSBs) and their repair are linked to epigenetic alterations.
  • DSBs are hypothesized to cause physiological dysfunction through cell-intrinsic and non-autonomous pathways.
  • Controlled DSB induction in vivo has been a significant challenge.

Purpose of the Study:

  • To develop a mouse model for spatiotemporal control of DSB induction at specific genomic loci.
  • To investigate the impact of DSBs on gene expression and epigenetic regulation in primary cells.
  • To determine the role of DSBs in cell-autonomous dysfunction and tissue maintenance.

Main Methods:

  • Development of a novel mouse model for inducible and tissue-specific DSB formation at targeted genomic sites.
  • Analysis of gene expression changes in primary cells following DSB induction and repair.
  • Assessment of the relationship between gene expression restoration, cell cycle progression, and DNA damage signaling.

Main Results:

  • DSBs induce a DNA damage signaling-dependent decrease in gene expression at break sites, which is reversible upon repair.
  • Gene expression restoration after DSB repair is independent of cell cycle progression.
  • Continuous DSB formation and repair in vivo did not lead to persistent transcriptional repression in lymphocytes.

Conclusions:

  • Primary cells possess a robust capacity to maintain transcriptome integrity despite DSBs.
  • DSB-induced transcriptional changes are transient and repair-dependent.
  • DNA damage plays a limited role in mediating cell-autonomous epigenetic dysfunction.