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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR01:59

CRISPR

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Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

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The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
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Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

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The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
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Updated: Mar 28, 2026

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
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Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

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Multiple Gene Repression in Cyanobacteria Using CRISPRi.

Lun Yao1, Ivana Cengic1, Josefine Anfelt1

  • 1KTH-Royal Institute of Technology , Division of Proteomics and Nanobiotechnology, Science for Life Laboratory, Stockholm SE-171 21 Sweden.

ACS Synthetic Biology
|December 23, 2015
PubMed
Summary
This summary is machine-generated.

We demonstrate clustered regularly interspaced short palindromic repeats interference (CRISPRi) for effective gene repression in cyanobacteria. This powerful tool enables precise control over gene expression for research and biotechnology applications.

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
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Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

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Related Experiment Videos

Last Updated: Mar 28, 2026

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • The model cyanobacterium Synechcocystis sp. PCC 6803 is a key organism for studying photosynthesis and biofuel production.
  • Gene repression is crucial for understanding gene function and metabolic engineering in cyanobacteria.

Discussion:

  • Clustered regularly interspaced short palindromic repeats interference (CRISPRi) was successfully applied for gene silencing in Synechcocystis.
  • Nuclease-deficient Cas9 enabled significant repression of green fluorescent protein (GFP) and carbon storage compounds (PHB, glycogen).
  • Simultaneous knockdown of multiple genes (aldehyde reductases/dehydrogenases) highlights CRISPRi's versatility for complex genetic studies.

Key Insights:

  • CRISPRi provides a robust method for achieving gene repression in cyanobacteria.
  • Inducible and reversible gene silencing is achievable using tightly repressed promoters with CRISPRi.
  • This technology facilitates both fundamental research and applied biotechnology in cyanobacteria.

Outlook:

  • CRISPRi opens new avenues for metabolic engineering of cyanobacteria for sustainable chemical production.
  • Further applications include precise functional genomics studies and synthetic biology approaches.
  • The development of inducible and reversible CRISPRi systems enhances its utility for dynamic biological pathway control.