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Spatiotemporal Expression of p63 in Mouse Epidermal Commitment.

Qian Zhao1, Shuang Liu2, Huishan Zhang3

  • 1State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. bingxia0923@163.com.

International Journal of Molecular Sciences
|December 23, 2015
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Summary

p63 expression precedes epidermal commitment in mouse embryos, appearing early and expanding across the surface. This study clarifies p63

Keywords:
K5/K14K8epidermal commitmentp63spatiotemporal expression pattern

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Embryonic surface ectoderm transitions from K8/K18 to K5/K14 expression during epidermal commitment.
  • The transcription factor p63 is known to be crucial for epidermal commitment, but its precise spatiotemporal expression during early mouse development is not fully understood.

Purpose of the Study:

  • To systematically investigate the expression pattern of p63 during early mouse epidermal commitment.
  • To correlate p63 expression with cytokeratin K8 and K5 expression during this developmental stage.

Main Methods:

  • Immunohistochemical analysis of p63, K8, and K5 expression in mouse embryos at various developmental stages.
  • Characterization of p63 isoforms, focusing on ΔNp63.

Main Results:

  • p63 expression is detectable as early as embryonic day 8.5 (E8.5), preceding epidermal commitment.
  • p63 expression initiates near somites and the posterior embryo, subsequently spreading across the embryonic surface, with notable enrichment in branchial arches and limb buds.
  • The ΔNp63 isoform is the predominant form during this period, and p63 expression intensity varies with embryonic location.

Conclusions:

  • A sequential and organized expression pattern of K8, p63, and K5 marks mouse epidermal commitment.
  • This research enhances the understanding of early epidermal development and provides a foundational resource for studying p63 function in mammals.