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Micromachined Fused Silica Liquid Core Waveguide Capillary Flow Cell.

K G Kraiczek1,2, J Mannion3, S Post3

  • 1Agilent Technologies , Hewlett-Packard Strasse 8, D 76337 Waldbronn, Germany.

Analytical Chemistry
|December 23, 2015
PubMed
Summary

This study presents a chip-based flow cell for UV-Vis absorbance detection in High-Performance Liquid Chromatography (HPLC). The device uses a microfabricated liquid core waveguide (LCW) for sensitive detection, with optimized geometries showing promising linearity.

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Area of Science:

  • Analytical Chemistry
  • Optical Engineering
  • Materials Science

Background:

  • High-Performance Liquid Chromatography (HPLC) requires sensitive detection methods.
  • Traditional flow cells can have limitations in path length and sensitivity.
  • Microfabrication offers potential for miniaturized and integrated optical detection systems.

Purpose of the Study:

  • To develop and characterize a planar, chip-based flow cell for UV-Vis absorbance detection in HPLC.
  • To investigate the performance of microfabricated liquid core waveguides (LCWs) for long path length detection.
  • To analyze the impact of waveguide geometry on linearity and calibration slope.

Main Methods:

  • Microfabrication of free-standing liquid core waveguide (LCW) capillary detection tubes.
  • Utilization of total internal reflection for light propagation within the LCW.
  • 3D ray tracing simulations to model waveguide behavior.
  • Experimental evaluation of linearity, calibration slope, and surface quality effects.

Main Results:

  • Lithographically produced LCWs with varying geometries were fabricated and tested.
  • 3D ray tracing was crucial for understanding complex waveguide geometries and multipath behavior.
  • Nonlinearity was observed but found to be manageable in specific, easily producible geometries.
  • Experimental performance correlated with LCW surface quality and light coupling efficiency.

Conclusions:

  • The developed planar, chip-based flow cell with LCW technology is a viable option for UV-Vis absorbance detection in HPLC.
  • Careful selection of LCW geometry can mitigate nonlinearity issues.
  • Surface quality and efficient light coupling are critical for optimal experimental performance.