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Updated: Mar 28, 2026
Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry
Published on: April 29, 2020
Production of Brazilian human norovirus VLPs and comparison of purification methods
Thais Alves da Costa Lamounier1, Layssa Miranda de Oliveira2, Brenda Rabello de Camargo2
1Departamento de Farmácia, Universidade de Brasília, Brasília, DF, Brazil.
Abstract:
Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
