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MicroRNA-495 suppresses human renal cell carcinoma malignancy by targeting SATB1

Cai Lv1, Zhiming Bai2, Zhenxiang Liu2

  • 1Department of Urology, Haikou Municipal Hospital Haikou, Hainan, 570208, China ; Department of Urology, Renmin Hospital of Wuhan University Wuhan, Hubei, 430060, China.

American Journal of Translational Research
|December 23, 2015
PubMed

Insights

MicroRNA-495 (miR-495) is downregulated in renal cell carcinoma (RCC). Restoring miR-495 suppresses RCC cell growth and migration by targeting SATB1, suggesting its potential as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
  • The role of miR-495 in renal cell carcinoma (RCC) remains largely unexplored.

Purpose of the Study:

  • To investigate the expression and function of miR-495 in RCC.
  • To identify potential targets of miR-495 in RCC.

Main Methods:

  • Quantitative real-time PCR to assess miR-495 expression in RCC cell lines and tissues.
  • Cell proliferation, migration assays, and cell cycle analysis to evaluate miR-495 function.
  • Luciferase reporter assays and Western blotting to validate SATB1 as a direct target of miR-495.

Main Results:

  • miR-495 expression was significantly downregulated in RCC cell lines and tissues compared to normal controls.
  • Ectopic expression of miR-495 induced G0/G1 phase arrest, suppressed cell proliferation, and inhibited cell migration in RCC cells.
  • SATB1 was identified as a direct target of miR-495 in RCC.
  • Re-expression of SATB1 partially reversed the inhibitory effects of miR-495 on cell proliferation and migration.

Conclusions:

  • miR-495 acts as a tumor suppressor in renal cell carcinoma.
  • miR-495 may serve as a potential diagnostic biomarker and therapeutic target for RCC.
Keywords:
Renal cell carcinomaSATB1miR-495microRNAs

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