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Related Experiment Video

Updated: Mar 28, 2026

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Published on: July 16, 2021

Fluorescence Phenomena in Nerve-Labeling Styryl-Type Dyes.

Tiberiu M Siclovan¹, Rong Zhang¹, Victoria Cotero¹

¹GE Global Research, One Research Circle, Niskayuna NY 12309.

Journal of Photochemistry and Photobiology. A, Chemistry

|December 23, 2015

Summary

This summary is machine-generated.

Researchers developed novel red-fluorescent styryl dyes with drug-like properties for biological imaging. Certain dyes exhibited dual emission, enabling selective nerve tissue staining and in-vivo imaging in rodents.

Keywords:

Styryl dyes TICT emission dyes fluorescence imaging of nerves in vivo and ex vixo imaging laparoscopy nerve labeling

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Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

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Published on: April 19, 2012

Area of Science:

Organic Chemistry
Photophysics
Biomedical Imaging

Background:

Styryl dyes are valuable fluorescent probes.
Extending fluorescence to the red spectrum is challenging while maintaining drug-like properties.
Understanding photophysical behavior is crucial for developing effective imaging agents.

Purpose of the Study:

Synthesize novel styryl dyes with red-shifted fluorescence.
Investigate their photophysical properties, including anomalous behaviors.
Evaluate their potential for nerve tissue staining and in-vivo imaging.

Main Methods:

Synthesis of diverse styryl dyes with varying donor-acceptor groups and conjugation lengths.
Characterization of optical and photophysical properties (absorption, emission, quantum yield).
Evaluation of nerve binding affinity and fluorescent staining of tissue sections.
In-vivo imaging studies in rodents using specialized instruments.

Main Results:

Several styryl dyes were synthesized, exhibiting tunable optical properties.
Anomalous photophysical behavior, including dual emission from ground state conformers (LE and TICT states), was observed in specific push-pull systems.
Compounds , , and showed robust nerve tissue staining.
Selected dyes were successfully used for in-vivo fluorescence imaging in rodents.

Conclusions:

Novel styryl dyes with red-shifted fluorescence and drug-like properties were developed.
Understanding structure-property relationships, including TICT phenomena, is key for dye design.
The selected dyes are promising tools for neuroimaging applications.