Purification of Na,K-ATPase from Pig Kidney
1Department of Biomedicine, Aarhus University, Ole Worms Allé 6, bldg. 1185, DK-8000, Aarhus C, Denmark. nf@biomed.au.dk.
Methods in Molecular Biology (Clifton, N.J.)
|December 24, 2015
Abstract:
The method of purification of Na,K-ATPase from pig kidney is based on a differential centrifugation and SDS-treatment of a microsomal preparation. The yield is 0.4 mg protein per 1 g tissue with the specific (ouabain-sensitive) activity of 25-28 μmol Pi/min per mg protein and nucleotide binding capacity of 3 nmol/mg. The protein/lipid ratio is 1/1 (mg/mg) with a protein purity of ~80 %.
