Related Experiment Video
Updated: Mar 28, 2026

Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle
Published on: March 21, 2025
Overproduction of PIB-Type ATPases
Xiangyu Liu1, Oleg Sitsel2, Kaituo Wang3
1School of Medicine, Tsinghua University, Beijing, China.
Abstract:
Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.
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