Simultaneous determination of three species with a single-injection step using batch injection analysis with multiple...

Area of Science:

• Analytical Chemistry
• Electrochemistry

Background:

• Simultaneous determination of multiple compounds in pharmaceutical samples presents analytical challenges.
• Existing methods may require complex procedures or multiple analytical steps.

Purpose of the Study:

• To demonstrate the first-time simultaneous determination of three compounds using batch injection analysis with multiple pulse amperometric detection (BIA-MPA).
• To develop a selective, rapid, and cost-effective analytical method for pharmaceutical analysis.

Main Methods:

• Utilized batch injection analysis (BIA) combined with multiple pulse amperometric detection (MPA).
• Applied a sequence of three potential pulses (+1.25 V, +1.60 V, +1.80 V) to generate three distinct amperograms.
• Employed current subtraction between amperograms with correction factors for selective compound determination.

Main Results:

• Achieved selective detection of 8-chlorotheophylline at +1.25 V.
• Enabled detection of 8-chlorotheophylline and pyridoxine at +1.60 V.
• Allowed simultaneous detection of 8-chlorotheophylline, pyridoxine, and diphenhydramine at +1.80 V.
• Demonstrated selective determination of pyridoxine and diphenhydramine through current subtraction.
• Method achieved high sample throughput (60 injections/hour).

Conclusions:

• The proposed BIA-MPA method enables the first simultaneous determination of three compounds in a single injection.
• The method is simple, inexpensive, fast, and selective for analyzing pharmaceutical samples.
• Results obtained are comparable to high-performance liquid chromatography (HPLC) at a 95% confidence level.