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Updated: Mar 28, 2026

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
Published on: November 16, 2016
Quantitative Packaging of Active Enzymes into a Protein Cage
Yusuke Azuma1, Reinhard Zschoche1, Matthias Tinzl1
1Laboratory of Organic Chemistry, ETH Zurich, 8093, Zurich, Switzerland.
Abstract:
Genetic fusion of cargo proteins to a positively supercharged variant of green fluorescent protein enables their quantitative encapsulation by engineered lumazine synthase capsids possessing a negatively charged lumenal surface. This simple tagging system provides a robust and versatile means of creating hierarchically ordered protein assemblies for use as nanoreactors. The generality of the encapsulation strategy and its effect on enzyme function were investigated with eight structurally and mechanistically distinct catalysts.
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