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Effect of ultrasound on the interleukin content in blood of rats with experimental inflammation
1Taras Shevchenko National University of Kiev, 60, Volodymyrska str., Кyiv, 01033, Ukraine.
Unlabelled:
The aim was to determine the effectiveness of anti-inflammatory action of ultrasound by the interleukin-6, interleukin-8 and tumor necrosis factor alpha content in the blood of rats with experimental inflammation.
Materials And Methods:
the study was performed using conventional models of inflammation - carrageenan-induced edema of the limbs of rats. Content of interleukins IL-6 and IL-8 was determined by enzyme immunoassay (ELISA) according to a standard protocol Immunoassay kit IL-10, Biosourse (USA). The content of TNFα was determined by cytotoxic effect on sensitive mouse fibroblast line L-929.
Results And Discussion:
it was shown that in the group of rats with carrageenan-induced edema contents of IL-6 increase in 2.8 times, IL-8 - in 5.6 times, and TNF-α - in 3 times compared to the control. Course influence of ultrasound contributed to reduction of the studied parameters. The content of IL-6 decreased in 1.7 times, IL-8 and TNF-α - in 1.6 times in comparison with inflammation.
