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Related Experiment Videos

18-Hydroxycorticosterone as a marker for primary hyperaldosteronism.

R Lapworth1, F Short, V H James

  • 1Department of Chemical Pathology, St Mary's Hospital, London, UK.

Annals of Clinical Biochemistry
|May 1, 1989
PubMed
Summary

A new assay accurately measures 18-hydroxycorticosterone (18-OHB) in plasma. Patients with primary hyperaldosteronism show elevated 18-OHB, with distinct postural responses differentiating adenomas from hyperplasia.

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Area of Science:

  • Endocrinology
  • Clinical Chemistry
  • Assay Development

Background:

  • Primary hyperaldosteronism is a condition characterized by excessive aldosterone production.
  • Accurate measurement of 18-hydroxycorticosterone (18-OHB) is crucial for diagnosing and managing primary hyperaldosteronism.
  • Existing diagnostic methods may have limitations in differentiating subtypes of the condition.

Purpose of the Study:

  • To validate a novel assay for quantifying 18-hydroxycorticosterone (18-OHB) in human plasma.
  • To assess the utility of the 18-OHB assay in distinguishing patients with primary hyperaldosteronism from healthy controls.
  • To investigate the potential of the assay to differentiate between unilateral adenomas and bilateral adrenal hyperplasia.

Main Methods:

  • Plasma samples were subjected to dichloromethane extraction.

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  • Separation of compounds was achieved using thin layer chromatography.
  • Quantification was performed via radioimmunoassay utilizing an iodinated 18-hydroxycorticosterone-3-carboxymethyloxime ligand.

    • Main Results:

    • The validated assay demonstrated reliable measurement of plasma 18-OHB.
    • Significantly higher mean plasma 18-OHB concentrations were observed in patients with primary hyperaldosteronism compared to controls.
    • Postural testing revealed an anomalous decrease in 18-OHB in patients with adenomas, contrasting with an increase in those with hyperplasia.

    Conclusions:

    • The developed radioimmunoassay provides a validated method for plasma 18-OHB measurement.
    • Elevated 18-OHB levels are indicative of primary hyperaldosteronism.
    • The distinct postural response patterns of 18-OHB may aid in differentiating adrenal adenomas from bilateral adrenal hyperplasia.