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Related Experiment Video

Updated: Mar 28, 2026

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Published on: November 15, 2019

Weaning Markedly Affects Transcriptome Profiles and Peyer's Patch Development in Piglet Ileum.

Ryo Inoue¹, Takamitsu Tsukahara², Masako Nakatani³

¹Laboratory of Animal Science, Kyoto Prefectural University , Kyoto , Japan ; Gut Immunology Group, Rowett Institute of Nutrition and Health, University of Aberdeen , Aberdeen , UK.

Frontiers in Immunology

|December 24, 2015

Summary

Early weaning significantly impacts piglet gut development, particularly Peyer

Keywords:

DNA microarray Peyer’s patches ileal mucosa metacore pathway analysis neonatal piglets transcriptome weaning

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Published on: February 10, 2023

Area of Science:

Animal Science
Immunology
Genomics

Background:

Postnatal gut development is crucial for piglet health and immunity.
Weaning is a critical transition period that can significantly alter gut physiology and immune function.

Purpose of the Study:

To investigate the effects of postnatal development and weaning on the piglet ileal mucosa transcriptome.
To identify key genes and pathways involved in gut development and immune response.

Main Methods:

Transcriptome analysis of ileal mucosa from piglets aged 14 to 35 days.
Comparison of gene expression profiles between suckling and weaned piglets.
Analysis of biological pathways, including leukocyte chemotaxis and immunity-related pathways.

Main Results:

Intensive gut development occurs between 14 and 21 postnatal days, with a higher number of differentially expressed genes.
Leukocyte chemotaxis pathway is significantly affected, showing different regulation in suckling versus weaned piglets.
Weaning negatively impacts acquired immunity pathways but positively affects innate immunity pathways.
Genes CXCL13, SLA-DOA, ICAM1, VAV1, and VCAM1, involved in leukocyte chemotaxis, declined postweaning and correlated with smaller Peyer's patches (PP).
Early weaning (14 days) caused irreversible adverse effects on PP development.

Conclusions:

The first 14-21 days are critical for piglet gut development.
Leukocyte chemotaxis genes are important for Peyer's patches development and are sensitive to weaning stress.
Early weaning significantly impairs gut immune development, with long-lasting consequences on Peyer's patches size and function.