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Updated: Mar 28, 2026

Isolation of Small Noncoding RNAs from Human Serum
Published on: June 19, 2014
MicroRNA expression profiles of serum from patients before and after chemotherapy
Yvonne Diener1, Thomas Walenda2, Edgar Jost3
1Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
Abstract:
Recovery of the blood and immune system after chemotherapy requires proliferation of hematopoietic stem and progenitor cells (HPSCs). It has been shown that systemically released factors in serum after chemotherapy stimulate HSPC expansion in vitro. We wondered if microRNAs (miRNAs) circulating in serum could account for this effect. Therefore, we compared the miRNA expression profiles of serum from patients with hematologic malignancies before and after chemotherapy. In addition to a general decrease in miRNA expression after chemotherapy, we found 23 miRNAs to be significantly differentially expressed in serum before versus after chemotherapy. The miRNA microarray data are available at NCBI's Gene Expression Omnibus (GEO) Series accession number GSE57570. Here, we provide a detailed protocol of the miRNA microarray and data analysis.
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