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A specific inhibition of induction of adaptive response by o-vanillin, a potent comutagen
K Takahashi1, M Sekiguchi, Y Kawazoe
1Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Biochemical and Biophysical Research Communications
|August 15, 1989
Abstract:
The present study revealed that potent comutagenic activity of o-vanillin in mutagenesis by N-methyl-N-nitrosourea (MNU) is a consequence of inhibition of the transcriptional promoting capacity of methylated O6-methylguanine-DNA methyltransferase (MGTase). As evidence of this, in the presence of o-vanillin, there were (i) dose-dependent decreases in MGTase induced in E. coli exposed to MNU, (ii) inhibition of ada gene promotion determined in the ada'-lacZ' assay system, and (iii) minimal inhibition of the methyl transfer capacity of MGTase in a subcellular system.