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Updated: Mar 28, 2026
Isolation and Derivation of Mouse Embryonic Germinal Cells
Published on: October 22, 2009
Isolation and Characterization of Fetal Leydig Progenitor Cells of Male Mice
Miki Inoue1, Yuichi Shima1, Kanako Miyabayashi1
1Division of Molecular Life Science (M.I., Y.S., T.B., K.-i.M.), Graduate School of Systems Life Science; Department of Molecular Biology (Y.S., K.M., K.T., T.B., K.-i.M.), Graduate School of Medical Sciences; Division of Bioinformatics (T.S., M.S.), Medical Institute of Bioregulation; and Department of Advanced Medical Initiatives (Y.O.), Japan Science and Technology Agency-Core Research for Evolutional Science and Technology, Faculty of Medicine, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan; and Department of Orthopaedics (H.A.), Gifu University Graduate School of Medicine, Gifu 501-1194, Japan.
Abstract:
Fetal and adult Leydig cells develop in mammalian prenatal and postnatal testes, respectively. In mice, fetal Leydig cells (FLCs) emerge in the interstitial space of the testis at embryonic day 12.5 and thereafter increase in number, possibly through differentiation from progenitor cells. However, the progenitor cells have not yet been identified. Previously, we established transgenic mice in which FLCs are labeled strongly with enhanced green fluorescent protein (EGFP). Interestingly, fluorescence-activated cell sorting provided us with weakly EGFP-labeled cells as well as strongly EGFP-labeled FLCs. In vitro reconstruction of fetal testes demonstrated that weakly EGFP-labeled cells contain FLC progenitors. Transcriptome from the 2 cell populations revealed, as expected, marked differences in the expression of genes required for growth factor/receptor signaling and steroidogenesis. In addition, genes for energy metabolisms such as glycolytic pathways and the citrate cycle were activated in strongly EGFP-labeled cells, suggesting that metabolism is activated during FLC differentiation.
