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MicroRNA expression profiles in pediatric dysembryoplastic neuroepithelial tumors
M Braoudaki1,2, G I Lambrou3, S A Papadodima4
1University Research Institute for the Study and Treatment of Childhood Genetic and Malignant Diseases, "Aghia Sophia" Children's Hospital, University of Athens, Athens, Greece. mbraoudak@med.uoa.gr.
Medical Oncology (Northwood, London, England)
|December 25, 2015
Summary
MicroRNAs (miRNAs) show diagnostic value in pediatric dysembryoplastic neuroepithelial tumors (DNETs). miR-3138 may suppress tumors, while miR-1909* may promote them, serving as potential DNET biomarkers.
Area of Science:
- Neuroscience
- Molecular Biology
- Oncology
Background:
- MicroRNAs (miRNAs) are critical noncoding RNAs in central nervous system pathologies.
- Pediatric dysembryoplastic neuroepithelial tumors (DNETs) require reliable diagnostic markers.
Purpose of the Study:
- To evaluate the diagnostic value of specific miRNAs in pediatric DNETs.
- To identify potential miRNA biomarkers for DNET diagnosis.
Main Methods:
- miRNA microarrays analyzing 1211 miRNAs in five pediatric DNETs and 17 control samples.
- Quantitative real-time polymerase chain reaction (qRT-PCR) for validating miR-1909* and miR-3138 expression.
Main Results:
- miR-3138 was down-regulated in DNETs, suggesting a tumor suppressor role.
- miR-1909* was up-regulated in DNETs, indicating a potential oncogenic function.
- Distinct miRNA expression profiles were observed between pediatric DNETs and controls.
Conclusions:
- miR-3138 and miR-1909* signatures may serve as diagnostic biomarkers for pediatric DNETs.
- These findings contribute to understanding miRNA involvement in DNET pathogenesis.
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