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MicroRNA expression profiles in pediatric dysembryoplastic neuroepithelial tumors

M Braoudaki1,2, G I Lambrou3, S A Papadodima4

  • 1University Research Institute for the Study and Treatment of Childhood Genetic and Malignant Diseases, "Aghia Sophia" Children's Hospital, University of Athens, Athens, Greece. mbraoudak@med.uoa.gr.

Medical Oncology (Northwood, London, England)
|December 25, 2015
PubMed

Insights

MicroRNAs (miRNAs) show diagnostic value in pediatric dysembryoplastic neuroepithelial tumors (DNETs). miR-3138 may suppress tumors, while miR-1909* may promote them, serving as potential DNET biomarkers.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Oncology

Background:

  • MicroRNAs (miRNAs) are critical noncoding RNAs in central nervous system pathologies.
  • Pediatric dysembryoplastic neuroepithelial tumors (DNETs) require reliable diagnostic markers.

Purpose of the Study:

  • To evaluate the diagnostic value of specific miRNAs in pediatric DNETs.
  • To identify potential miRNA biomarkers for DNET diagnosis.

Main Methods:

  • miRNA microarrays analyzing 1211 miRNAs in five pediatric DNETs and 17 control samples.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) for validating miR-1909* and miR-3138 expression.

Main Results:

  • miR-3138 was down-regulated in DNETs, suggesting a tumor suppressor role.
  • miR-1909* was up-regulated in DNETs, indicating a potential oncogenic function.
  • Distinct miRNA expression profiles were observed between pediatric DNETs and controls.

Conclusions:

  • miR-3138 and miR-1909* signatures may serve as diagnostic biomarkers for pediatric DNETs.
  • These findings contribute to understanding miRNA involvement in DNET pathogenesis.
Keywords:
BiomarkersBrain tumorsMicroRNA microarraysPediatricROC curvesqRT-PCR

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