Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

10.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.2K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interlaboratory Comparison of a Glucagon and Oxyntomodulin Immuno-LC-MS/MS Assay: Implications for Diabetes Research.

Clinical chemistry·2026
Same author

Proteome of Bronchial Alveolar Lavage fluid and Serum from Tuberculosis patients during drug treatment.

Scientific data·2026
Same author

Circulating protein biomarkers of physical fitness associated with cardiometabolic risk in women after gestational diabetes: a PONCH study.

Cardiovascular diabetology·2026
Same author

Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.

Nature chemical biology·2026
Same author

Targeting α v β 8 Integrin with mAb MEDI8367 Prevents Fibrosis in Preclinical Models of CKD.

Kidney360·2025
Same author

Enhancing CHO cell recombinant protein production using a perfusion-directed host evolution approach.

Biotechnology progress·2025

Related Experiment Video

Updated: Mar 28, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.1K

A Targeted MRM Approach for Tempo-Spatial Proteomics Analyses.

Annie Moradian1, Tanya R Porras-Yakushi1, Michael J Sweredoski1

  • 1Proteome Exploration Laboratory, California Institute of Technology, BI 211, MC139-74, Pasadena, CA, 91125, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 25, 2015
PubMed
Summary

This study presents a generic protocol for developing a multiple reaction monitoring (MRM) assay. This targeted proteomics technique offers high selectivity, sensitivity, and reproducibility for protein quantification.

Keywords:
MRMQuadrupole mass spectrometryQuantitation

More Related Videos

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

13.5K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.5K

Related Experiment Videos

Last Updated: Mar 28, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.1K
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

13.5K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.5K

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Quantitative proteomics requires high selectivity, sensitivity, and reproducibility.
  • Discovery-based proteomics often faces challenges that targeted approaches can overcome.
  • Multiple Reaction Monitoring (MRM) is a powerful targeted proteomics technique.

Purpose of the Study:

  • To present a generic protocol for the development of an MRM assay.
  • To facilitate the application of targeted proteomics for protein quantification.
  • To provide a standardized method for MRM assay development.

Main Methods:

  • Utilizing established pharmaceutical industry guidelines for assay setup.
  • Synthesizing proteotypic peptides with heavy isotopes as internal standards.
  • Determining retention times and calibration curves using triple-quadrupole mass spectrometers.
  • Integrating iRT peptide standards into the bioinformatics pipeline.
  • Quantifying target proteins in digested biological samples mixed with standards.

Main Results:

  • A straightforward protocol for MRM assay development is presented.
  • The protocol ensures the use of peptides with favorable mass spectrometric properties.
  • The method allows for accurate quantification of protein targets.

Conclusions:

  • The developed protocol provides a reliable framework for MRM assay development.
  • This approach enhances the accuracy and reproducibility of quantitative proteomics.
  • The protocol is applicable for known protein targets in hypothesis-driven or discovery-driven research.