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Tissue MALDI Mass Spectrometry Imaging (MALDI MSI) of Peptides

Birte Beine1, Hanna C Diehl2, Helmut E Meyer1

  • 1Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Dortmund, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|December 25, 2015
PubMed

Insights

This study details protocols for matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) of peptides in tissues. It covers sample preparation for cryoconserved and FFPE tissues, MALDI measurement, and data analysis using specific devices.

Area of Science:

  • Mass Spectrometry Imaging
  • Proteomics
  • Molecular Imaging

Background:

  • Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) visualizes molecular distributions in tissues.
  • Peptide analysis using MALDI MSI requires optimized sample preparation and measurement protocols.

Purpose of the Study:

  • To provide detailed operational instructions for MALDI MSI of peptides.
  • To describe sample preparation for both cryoconserved and formalin-fixed paraffin-embedded (FFPE) tissues.
  • To outline protocols for MALDI measurement, tissue staining, and data analysis.

Main Methods:

  • Detailed protocols for cryoconserved and FFPE tissue sample preparation.
  • On-tissue digestion and matrix application procedures.
  • Description of MALDI measurement and data analysis steps.
  • Utilized SunCollect and ImagePrep spraying devices for matrix application.

Main Results:

  • Established comprehensive protocols for peptide MALDI MSI.
  • Demonstrated effective sample preparation for diverse tissue types.
  • Provided guidance on matrix application using specific commercial devices.

Conclusions:

  • The described protocols facilitate robust peptide MALDI MSI analysis.
  • These methods are applicable to both fresh-frozen and preserved tissue samples.
  • The study offers a valuable resource for researchers performing peptide imaging mass spectrometry.
Keywords:
CryoconservedFormalin-fixed paraffin-embedded (FFPE)ImagePrepMALDI imagingMSIMatrix applicationPeptideSunCollectTrypsin

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