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Calcium signalling in non-excitable cells: notes on oscillations and store refilling
1Smith Kline & French Research Ltd, The Frythe, Welwyn, UK.
Cell Calcium
|July 1, 1989
Summary
Recent advances enable measurement of intracellular calcium (Ca2+) in non-excitable cells, transforming cell signaling research. This breakthrough expands our understanding of cellular functions beyond muscle and nerve tissues.
Area of Science:
- Cell Biology
- Biochemistry
- Physiology
Background:
- Cellular calcium concentration ([Ca2+]i) is a critical signaling variable.
- Historically, [Ca2+]i measurement was limited to large invertebrate cells and excitable cells (muscle, nerve).
- Early techniques included aequorin, arsenazo III, Ca-selective micro-electrodes, and electropermeabilized cells.
Purpose of the Study:
- To review the transformation in studying cellular calcium signaling.
- To highlight the expansion of measurement capabilities to non-excitable cells.
- To underscore the impact of technical advances on signal transduction research.
Main Methods:
- Aequorin and arsenazo III for monitoring [Ca2+]i.
- Development of Ca-selective micro-electrodes.
- Use of electropermeabilized cells to define Ca2+ concentrations.
- Electrophysiology in excitable cells.
Main Results:
- Technical advances have revolutionized the study of cellular calcium.
- The ability to measure [Ca2+]i has been extended to non-excitable cells.
- Research has shifted from primarily excitable cells to a broader range of cell types.
Conclusions:
- The field of cellular calcium signaling has undergone significant transformation.
- New technical capabilities have broadened the scope of research.
- Understanding of signal transduction pathways is greatly enhanced.