Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

515
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
515
Stringent Response in E. coli01:23

Stringent Response in E. coli

466
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
466
Responses to Salt Stress02:02

Responses to Salt Stress

15.0K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
15.0K
RNA-seq03:21

RNA-seq

12.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.5K
RNA Stability01:53

RNA Stability

36.2K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
36.2K
RNA Stability01:53

RNA Stability

12.1K
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of the bacterial community of the human upper respiratory tract in patients affected by Covid-19.

Genetics and molecular biology·2026
Same author

The genome sequence of the açaí berry (<i>Euterpe oleracea</i> Mart.) and RNA-Seq analysis of the fruit ripening.

Genome·2026
Same author

Unveiling the Probiotic Properties of Lacticaseibacillus paracasei UFTM 2.9 Through Probiogenomic Analysis.

Molecular nutrition & food research·2026
Same author

PanGeneWhale - A dockerized Kotlin-based GUI platform for reproducible and user-friendly pangenomic analysis.

Computational biology and chemistry·2025
Same author

ContigPolishing: A User-Friendly Java GUI for contig extension and refinement in prokaryotic genomes.

Gene·2025
Same author

A Flexible Fluid Delivery System for Rodent Behavior Experiments.

eNeuro·2025

Related Experiment Video

Updated: Mar 28, 2026

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.5K

Corynebacterium pseudotuberculosis RNA-seq data from abiotic stresses.

Pablo H C G de Sá1, Adonney A O Veras1, Adriana R Carneiro1

  • 1Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil.

Data in Brief
|December 25, 2015
PubMed
Summary

Corynebacterium pseudotuberculosis adaptation mechanisms were studied under simulated host conditions. This research helps understand how the bacterium survives during infection in goats and sheep.

Keywords:
Corynebaterium pseudotuberculosisInfection stressesRNA-seqSOLiD

More Related Videos

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

7.6K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.9K

Related Experiment Videos

Last Updated: Mar 28, 2026

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.5K
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

7.6K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.9K

Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Host-Pathogen Interactions

Background:

  • Corynebacterium pseudotuberculosis is a significant pathogen causing caseous lymphadenitis in small ruminants.
  • The disease results in substantial economic losses for farmers due to animal morbidity and mortality.
  • Understanding bacterial adaptation is crucial for developing effective disease control strategies.

Purpose of the Study:

  • To investigate the gene expression profile of Corynebacterium pseudotuberculosis under simulated host-specific stress conditions.
  • To identify bacterial adaptation mechanisms relevant to the infectious process in goats and sheep.
  • To provide insights into the molecular basis of caseous lymphadenitis pathogenesis.

Main Methods:

  • Bacterial cultures of Corynebacterium pseudotuberculosis were subjected to osmotic, acid, and heat stress.
  • RNA was extracted from stressed and control (Normal-BHI) cultures.
  • cDNA libraries were prepared and sequenced using the SOLiD3 Plus platform with RNA-Seq technology.
  • Differential gene expression analysis was performed on the sequencing data.

Main Results:

  • Significant alterations in gene expression were observed under osmotic, acid, and heat stress conditions compared to control.
  • Specific genes and pathways involved in stress response and adaptation were identified.
  • The study revealed key molecular mechanisms employed by the bacterium to survive within the host environment.

Conclusions:

  • Corynebacterium pseudotuberculosis exhibits robust adaptive responses to environmental stresses encountered during infection.
  • Differential gene expression analysis provides valuable information for understanding the pathogenesis of caseous lymphadenitis.
  • These findings can inform the development of novel diagnostic and therapeutic interventions for controlling this economically important disease.