Nile Red Derivative-Modified Nanostructure for Upconversion Luminescence Sensing and Intracellular Detection of...

Iron ion (Fe(3+)) which is the physiologically most abundant and versatile transition metal in biological systems, has been closely related to many certain cancers, metabolism, and dysfunction of organs, such as the liver, heart, and pancreas. In this Research Article, a novel Nile red derivative (NRD) fluorescent probe was synthesized and, in conjunction with polymer-modified core-shell upconversion nanoparticles (UCNPs), demonstrated in the detection of Fe(3+) ion with high sensitivity and selectivity. The core-shell UCNPs were surface modified using a synthesized PEGylated amphiphilic polymer (C18PMH-mPEG), and the resulting mPEG modified core-shell UCNPs (mPEG-UCNPs) show good water solubility. The overall Fe(3+)-responsive upconversion luminescence nanostructure was fabricated by linking the NRD to the mPEG-UCNPs, denoted as mPEG-UCNPs-NRD. In the nanostructure, the core-shell UCNPs, NaYF4:Yb,Er,Tm@NaGdF4, serve as the energy donor while the Fe(3+)-responsive NRD as the energy acceptor, which leads to efficient luminescence resonance energy transfer (LRET). The mPEG-UCNPs-NRD nanostructure shows high selectivity and sensitivity for detecting Fe(3+) in water. In addition, benefited from the good biocompatibility, the nanostructure was successfully applied for detecting Fe(3+) in living cells based on upconversion luminescence (UCL) from the UCNPs. Furthermore, the doped Gd(3+) ion in the UCNPs endows the mPEG-UCNPs-NRD nanostructure with effective T1 signal enhancement, making it a potential magnetic resonance imaging (MRI) contrast agent. This work demonstrates a simple yet powerful strategy to combine metal ion sensing with multimodal bioimaging based on upconversion luminescence for biomedical applications.