Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Organization of Genes02:07

Organization of Genes

74.3K
Overview
74.3K
Organization of Genes02:07

Organization of Genes

18.2K
18.2K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.9K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.9K
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

54.0K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
54.0K
Nucleosome Remodeling02:54

Nucleosome Remodeling

11.6K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
11.6K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

25.2K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
25.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome organization by SATB1 binding to base-unpairing regions (BURs) provides a scaffold for SATB1-regulated gene expression.

eLife·2025
Same author

HMGA1 acts as an epigenetic gatekeeper of ASCL2 and Wnt signaling during colon tumorigenesis.

The Journal of clinical investigation·2025
Same author

An Optimized Adaptation of DamID for NGS Applications.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Lamins: The backbone of the nucleocytoskeleton interface.

Current opinion in cell biology·2024
Same author

Choreography of lamina-associated domains: structure meets dynamics.

FEBS letters·2023
Same author

Nucleoplasmic lamin C rapidly accumulates at sites of nuclear envelope rupture with BAF and cGAS.

The Journal of cell biology·2022

Related Experiment Video

Updated: Mar 28, 2026

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
11:25

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

11.0K

Finding the Middlemen in Genome Organization.

Xianrong Wong1, Karen L Reddy1

  • 1Department of Biological Chemistry, Center for Epigenetics, Johns Hopkins University, 855 N. Wolfe Street, Baltimore, MD 21205, USA.

Developmental Cell
|December 26, 2015
PubMed
Summary
This summary is machine-generated.

Researchers found a protein linking repressive chromatin to the inner nuclear membrane, explaining how silent genetic material is anchored at the cell

More Related Videos

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
09:14

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

20.3K
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

33.7K

Related Experiment Videos

Last Updated: Mar 28, 2026

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
11:25

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

11.0K
DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
09:14

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

20.3K
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

33.7K

Area of Science:

  • Cell Biology
  • Epigenetics
  • Molecular Biology

Background:

  • Chromatin domains near the nuclear lamina are typically heterochromatic and transcriptionally silent.
  • The mechanisms for recruiting and maintaining these chromatin domains at the nuclear periphery are not well understood.

Purpose of the Study:

  • To identify the molecular link between repressive chromatin and the inner nuclear membrane.
  • To elucidate the role of specific proteins in anchoring heterochromatin to the nuclear periphery.

Main Methods:

  • The study utilized techniques to identify and characterize chromatin-binding proteins.
  • Investigated the interaction between identified proteins and components of the inner nuclear membrane.

Main Results:

  • A novel chromatin-binding protein was identified that specifically associates with repressive chromatin.
  • This protein was shown to bridge heterochromatic regions to the inner nuclear membrane.

Conclusions:

  • The identified protein serves as a crucial link, anchoring transcriptionally repressed chromatin to the nuclear periphery.
  • This finding provides insight into the spatial organization of the genome and the maintenance of heterochromatin.