Streptomyces yangpuensis sp. nov., isolated from soil

A novel Gram-stain-positive strain with sandy aerial mycelium and golden yellow substrate mycelium, designated fd2-tb^T, was isolated from a soil sample collected in Shanghai, China, and its taxonomic status was established by phylogenetic analysis. 16S rRNA gene sequence analysis showed that strain fd2-tb^T belonged to the genus Streptomyces and was related to Streptomyces amritsarensis JCM 19660^T (99.9 % 16S rRNA gene sequence similarity), Streptomyces flavotricini NBRC 12770^T (99.9 %), Streptomyces polychromogenes NBRC 13072^T (99.8 %), Streptomyces racemochromogenes NRRL B-5430^T (99.7 %), Streptomyces globosus LMG 19896^T (99.5 %), Streptomyces toxytricini NBRC 12823^T (99.5 %) and Streptomyces katrae NBRC 13447^T (99.3 %). The cell wall of strain fd2-tb^T contained ll-diaminopimelic acid, and whole-cell sugars were identified as glucose and ribose. The menaquinones MK-9(H₄), MK-9(H₆) and MK-9(H₈) were also detected. In addition, the polar lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, as well as five unidentified phospholipids, were detected. Major cellular fatty acids were identified as anteiso-C_15 : 0, iso-C_16 : 0, anteiso-C_17 : 0, iso-C_15 : 0 and C_16 : 0. DNA-DNA hybridization experiments showed that strain fd2-tb^T exhibited 36.5 ± 0.6 %, 43.5 ± 2.0 %, 11.1 ± 1.3 %, 10.3 ± 3.1 %, 9.8 ± 1.9 %, 48.9 ± 3.9 % and 16.3 ± 1.7 % relatedness to S. amritsarensis JCM 40119660^T, S. flavotricini NBRC 12770^T, S. polychromogenes NBRC 13072^T, S. racemochromogenes NRRL B-5430^T, S. globosus LMG 19896^T, S. toxytricini NBRC 12823^T and S. katrae NBRC 13447^T, respectively. Based on these analyses as well as some phenotypic differences, strain fd2-tb^T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces yangpuensis sp. nov. is proposed. The type strain is fd2-tb^T ( = DSM 100336^T = CGMCC 4.7256^T).