Biomarker discovery in mass spectrometry-based urinary proteomics

Area of Science:

• Clinical bioanalysis
• Proteomics
• Biomarker discovery

Background:

• Urinary proteomics is a key area for identifying disease biomarkers.
• Mass spectrometry (MS)-based proteomic analysis is a powerful tool in clinical bioanalysis.
• Few protein biomarkers have progressed to clinical validation due to challenges like urine complexity and wide dynamic protein ranges.

Purpose of the Study:

• To review promising technologies and strategies for MS-based urinary biomarker discovery.
• To emphasize advancements in maximizing urinary proteome coverage and standardization.
• To discuss the potential of MS-based urinary proteomics for noninvasive diagnostic assays.

Main Methods:

• Review of study design, sample preparation, protein quantification, instrumental platforms, and bioinformatics in MS-based biomarker discovery.
• Discussion of different proteomics approaches.
• Emphasis on progress in urinary proteome coverage and standardization.

Main Results:

• Identification of promising technologies and strategies for MS-based urinary biomarker discovery.
• Highlighting progress in overcoming challenges in urine sample analysis.
• Demonstrating the potential for improved proteome coverage and standardization.

Conclusions:

• MS-based urinary proteomics holds significant potential for developing noninvasive diagnostic assays.
• Collaborative efforts among analytical scientists, systems biologists, and clinicians are crucial for future success.
• Continued advancements in technology and standardization are needed to translate research into clinical practice.