Related Experiment Video
Updated: Mar 28, 2026
Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection
Published on: September 21, 2014
[Long non-coding RNA Gm15577 is involved in mouse cerebellar neurogenesis]
Yongsong Yue1, Weilong Zhang, Chunying Liu
1Department of Pathology, School of Basic Medicine Peking Union Medical College; Neuroscience Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China.
Objective:
To identify novel lncRNAs involved in cerebellar neurogenesis using neuronal specific Nbs1-deficient (Nbs1(CNS-del)) mouse model.
Methods:
Microarray analysis was performed to identify differentially expressed lncRNAs between Nbs1(CNS-ctr) and Nbs1(CNS-del) mice. Expression profiles of lncRNA Gm15577 and coding gene Negr1 in mice, primary cerebellar culture and cell lines were measured using RT-qPCR. Subcellular fractionation was performed to determine the subcellular localization of Gm15577.
Results:
Gm15577 was specifically expressed in mice cerebellum in a developmentally regulated manner, which could be abolished upon Nbs1-deficiency. Gm15577 was located in the intronic region of Negr1 in a reversed orientation. Gm15577 modulated the RNA expression of Negr1, Shh and β-catenin. NEGR1 had a distinct expression pattern between normal and medulloblastoma patients.
Conclusion:
Gm15577 may modulate cerebellar granule cell proliferation and differentiation by targeting Negr1, and their dysfunctions or abnormal expression may be related to tumorigenesis of medulloblastoma.
