Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Metabolic States of the Body: Fasting and Starvation01:24

Metabolic States of the Body: Fasting and Starvation

3.3K
During the initial hours of fasting, the body uses up its glycogen stores as an energy source. Once these glycogen reserves are depleted, the body begins breaking down stored triglycerides and structural proteins. During this stage, glycerol becomes a key substrate for gluconeogenesis, while free fatty acids undergo beta-oxidation to provide energy for tissues, such as skeletal muscle. In the fasting state, the body spares protein breakdown as much as possible to conserve muscle and structural...
3.3K
Metabolic States of the Body: The Postabsorptive State01:18

Metabolic States of the Body: The Postabsorptive State

1.6K
The postabsorptive state usually starts about four hours after a meal and lasts until the next meal is eaten. During this time, the digestive system stops absorbing nutrients, and the body uses stored energy reserves to maintain stable blood glucose levels.
Initially, glycogen stored in the liver is broken down to release glucose into the bloodstream, while glycogen in the muscles is broken down to supply glucose for energy directly within the muscle cells. As glycogen stores diminish,...
1.6K
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

2.2K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
2.2K
Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

8.4K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Traffic State Lane-Level Estimation Based on Transformer and Vehicle Trajectory Data.

Sensors (Basel, Switzerland)·2026
Same author

Longitudinal localization of leukaemic stem cells between the metaphysis and central marrow governs their behaviour.

Nature cell biology·2026
Same author

Trajectory planning for traffic safety with dynamic ethical risk adjustment.

Accident; analysis and prevention·2026
Same author

Tumor-initiating stem cells engineer immunity.

Cancer cell·2025
Same author

A unified risk metric for freeway vehicles based on the spatio-temporal overlap probability of predicted positions.

Accident; analysis and prevention·2025
Same author

DCLK1-mediated regulation of invadopodia dynamics and matrix metalloproteinase trafficking drives invasive progression in head and neck squamous cell carcinoma.

Molecular cancer·2025

Related Experiment Video

Updated: Mar 28, 2026

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
11:03

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Published on: September 9, 2021

4.6K

Stem Cells Matter in Response to Fasting.

Badi Sri Sailaja1, Xi C He1, Linheng Li2

  • 1Stowers Institute for Medical Research, Kansas City, MO 64110, USA.

Cell Reports
|December 27, 2015
PubMed
Summary
This summary is machine-generated.

Intestinal stem cells remain dormant during fasting and are reactivated upon re-feeding. This study reveals that PTEN regulation is key to controlling intestinal stem cell activity based on nutritional status.

More Related Videos

Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells
06:33

Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells

Published on: February 2, 2024

2.8K
Analysis of Hematopoietic Stem Progenitor Cell Metabolism
12:20

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Published on: November 9, 2019

7.4K

Related Experiment Videos

Last Updated: Mar 28, 2026

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
11:03

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Published on: September 9, 2021

4.6K
Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells
06:33

Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells

Published on: February 2, 2024

2.8K
Analysis of Hematopoietic Stem Progenitor Cell Metabolism
12:20

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Published on: November 9, 2019

7.4K

Area of Science:

  • Gastroenterology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Intestinal adaptation to fasting and re-feeding involves complex molecular mechanisms.
  • The role of intestinal stem cells (ISCs) in this adaptive process is not fully understood.
  • Nutritional status significantly impacts gut physiology and cellular function.

Purpose of the Study:

  • To elucidate the molecular regulation of intestinal stem cells during fasting and re-feeding.
  • To identify key signaling pathways and proteins involved in ISC dormancy and activation.
  • To understand how nutritional cues influence ISC behavior.

Main Methods:

  • Utilized mouse models to study intestinal adaptation.
  • Employed genetic manipulation to investigate the role of PTEN in ISCs.
  • Analyzed ISC markers and proliferation rates under different nutritional conditions.
  • Performed molecular analyses to identify signaling pathways.

Main Results:

  • Identified PTEN as a critical regulator of intestinal stem cell dormancy during fasting.
  • Demonstrated that PTEN levels decrease upon re-feeding, promoting ISC activation.
  • Showcased the direct link between nutritional status and ISC behavior via PTEN signaling.
  • Revealed specific molecular pathways modulated by PTEN in ISCs.

Conclusions:

  • PTEN acts as a crucial gatekeeper for intestinal stem cell activity in response to nutrient availability.
  • Understanding PTEN's role provides insights into maintaining gut homeostasis during metabolic changes.
  • This discovery opens new avenues for therapeutic strategies targeting gut adaptation and repair.