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Related Experiment Videos

Gene function identified by interspecific transformation.

M E Katz1, M J Hynes

  • 1Department of Genetics, University of Melbourne, Parkville, Victoria, Australia.

Gene
|May 15, 1989
PubMed
Summary
This summary is machine-generated.

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Aspergillus nidulans utilizes 2-pyrrolidinone as a nitrogen source, unlike related species. Genes lamA and lamB from A. nidulans are essential for this utilization in Aspergillus terreus.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Aspergillus nidulans can metabolize 2-pyrrolidinone for nitrogen, but Aspergillus niger and Aspergillus terreus cannot.
  • Mutations in the lamA gene disrupt 2-pyrrolidinone utilization in A. nidulans.

Purpose of the Study:

  • To identify the genetic basis for 2-pyrrolidinone utilization in Aspergillus species.
  • To investigate the function of lamA and a novel adjacent gene in nitrogen source metabolism.

Main Methods:

  • Isolation of the lamA gene and an adjacent gene from A. nidulans using plasmid pLAM7.
  • Genetic transformation of Aspergillus terreus with subclones of pLAM7.
  • Assessing the ability of transformed A. terreus to utilize 2-pyrrolidinone.

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Main Results:

  • A. nidulans lamA and a previously unknown gene, designated lamB, were identified on plasmid pLAM7.
  • Transformation of A. terreus with both lamA and lamB restored the ability to utilize 2-pyrrolidinone.
  • Both lamA and lamB are essential for 2-pyrrolidinone utilization in A. terreus.

Conclusions:

  • The lamA and lamB genes are crucial for the utilization of 2-pyrrolidinone as a nitrogen source in Aspergillus.
  • These genes enable a metabolic pathway for 2-pyrrolidinone assimilation, which is absent in some related Aspergillus species.