Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

33.6K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
33.6K
Types of RNA01:23

Types of RNA

73.8K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
73.8K
Regulated mRNA Transport02:22

Regulated mRNA Transport

7.2K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
7.2K
Translational Regulation01:29

Translational Regulation

807
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
807

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

APEX2 proximity labeling of RNA in bacteria.

Cell reports methods·2025
Same author

A simple, fast, and cost-efficient protocol for ultra-sensitive ribosome profiling.

Nucleic acids research·2025
Same author

Optimized <i>Legionella</i> expression strain for affinity purification of His-tagged membrane proteins eliminates major multimeric contaminant.

Microbiology spectrum·2025
Same author

Uncovering the mechanism for polar sequestration of the major bacterial sugar regulator by high-throughput screens and 3D interaction modeling.

Cell reports·2025
Same author

Membrane association and polar localization of the <i>Legionella pneumophila</i> T4SS DotO ATPase mediated by two nonredundant receptors.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

APEX2 proximity labeling of RNA in bacteria.

bioRxiv : the preprint server for biology·2024

Related Experiment Video

Updated: Mar 28, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

9.6K

Methods for studying RNA localization in bacteria.

Shanmugapriya Kannaiah1, Orna Amster-Choder1

  • 1Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University - Faculty of Medicine, P.O. Box 12272, Jerusalem 91120, Israel.

Methods (San Diego, Calif.)
|December 29, 2015
PubMed
Summary
This summary is machine-generated.

Researchers explore bacterial RNA localization, revealing specific RNA targeting mechanisms even without a nucleus. This study details methods for studying RNA localization in bacteria, discussing their pros and cons.

Keywords:
Bacterial cellFluorescence microscopyImaging techniquesQuenchingRNA localizationSuper-resolution microscopy

More Related Videos

RNA Fluorescence in situ Hybridization FISH to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines
08:58

RNA Fluorescence in situ Hybridization FISH to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

Published on: July 27, 2022

5.2K
Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
07:51

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

8.8K

Related Experiment Videos

Last Updated: Mar 28, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

9.6K
RNA Fluorescence in situ Hybridization FISH to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines
08:58

RNA Fluorescence in situ Hybridization FISH to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

Published on: July 27, 2022

5.2K
Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
07:51

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

8.8K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Microbiology

Background:

  • Subcellular localization of RNA transcripts is crucial for understanding biological processes.
  • RNA targeting mechanisms are a key focus in modern cell biology.
  • Recent advancements in imaging and microscopy have revealed specific RNA localization in bacteria, despite their lack of a nucleus.

Purpose of the Study:

  • To describe methods for studying RNA localization in bacteria.
  • To discuss the applications, advantages, and limitations of these methods.
  • To provide insights into the mechanisms of bacterial RNA targeting.

Main Methods:

  • In situ hybridization techniques.
  • Live-cell imaging.
  • Advanced optical microscopy.

Main Results:

  • Bacterial RNAs exhibit specific subcellular localization.
  • Various imaging and microscopy techniques enable the study of RNA localization in bacteria.
  • The described methods offer different advantages and face specific limitations.

Conclusions:

  • Understanding bacterial RNA localization is essential for deciphering cellular functions.
  • The presented methods are valuable tools for investigating RNA targeting in prokaryotes.
  • Further research can build upon these techniques to explore bacterial RNA biology.