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Updated: Mar 28, 2026

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos

Published on: August 3, 2012

RHEB1 expression in embryonic and postnatal mouse.

Qi Tian¹, James L Smart², Joachim H Clement³

¹OHSU Transgenic Mouse Models Shared Resource, Knight Cancer Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR, 97239, USA.

Histochemistry and Cell Biology

|December 29, 2015

Summary

This summary is machine-generated.

Ras homolog enriched in brain (RHEB1) is crucial for embryonic development, showing ubiquitous expression. Postnatal RHEB1 expression becomes tissue-specific, particularly in neural and internal organs, suggesting diverse physiological roles.

Keywords:

Adult mice Embryos Expression pattern Gene trap Immunohistochemistry Rheb1 gene X-gal staining

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Last Updated: Mar 28, 2026

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Published on: June 26, 2018

Area of Science:

Molecular Biology
Developmental Biology
Genetics

Background:

Ras homolog enriched in brain (RHEB1) is a GTP-binding protein involved in critical signaling pathways.
RHEB1's role in physiological processes necessitates understanding its expression patterns.

Purpose of the Study:

To investigate the spatiotemporal expression pattern of RHEB1 during mouse embryonic and postnatal development.
To elucidate the physiological relevance of RHEB1 through its expression profile.

Main Methods:

Analysis of RHEB1 expression using immunostaining in wild-type mice.
X-gal staining in Rheb1 gene trap mutant mice to track RHEB1 expression.
Examination of expression from embryonic day 3.5 to adult stages.

Main Results:

Ubiquitous RHEB1 expression observed in early embryos (up to E12.5).
Complex, tissue-specific expression emerges in later embryonic stages (E13.5-E16.5), with high levels in neural tissues (brain, CNS) and peripheral organs (heart, muscle).
Postnatal mice show broad RHEB1 expression in the brain (cortex, hippocampus, cerebellum) and internal organs, with varying intensities indicating dynamic regulation.

Conclusions:

RHEB1 exhibits a ubiquitous role in early embryonic development.
RHEB1 expression becomes more specialized in later development, highlighting its diverse functions in various tissues and organs.
The complex expression pattern suggests RHEB1's involvement in multiple, tissue-specific physiological processes throughout life.