Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Yeast proteins that recognize nuclear localization sequences.

P Silver1, I Sadler, M A Osborne

  • 1Department of Biology, Princeton University, New Jersey 08544.

The Journal of Cell Biology
|September 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-architecture tuning of silicon and SiGe-based quantum devices using machine learning.

Scientific reports·2024
Same author

Machine learning enables completely automatic tuning of a quantum device faster than human experts.

Nature communications·2020
Same author

The arrangement of resources in patchy landscapes: effects on distribution, survival, and resource acquisition of chironomids.

Oecologia·2017
Same author

Correction: Charge-tunnelling and self-trapping: common origins for blinking, grey-state emission and photoluminescence enhancement in semiconductor quantum dots.

Nanoscale·2016
Same author

Charge-tunnelling and self-trapping: common origins for blinking, grey-state emission and photoluminescence enhancement in semiconductor quantum dots.

Nanoscale·2016
Same author

Hydration and endocrine responses to intravenous fluid and oral glycerol.

Scandinavian journal of medicine & science in sports·2015

Researchers found two yeast proteins that bind to nuclear localization signals, suggesting they act as receptors to direct proteins into the cell nucleus. This discovery aids in understanding nuclear transport mechanisms.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Trafficking

Background:

  • Nuclear localization signals (NLS) mediate protein transport into the nucleus.
  • Specific receptors are hypothesized to recognize and facilitate this process.

Purpose of the Study:

  • To identify and characterize proteins in yeast that bind to known nuclear localization peptides.
  • To elucidate the mechanism of nuclear protein import.

Main Methods:

  • Binding assays using peptide-albumin conjugates immobilized on nitrocellulose filters.
  • Fractionation of binding proteins with cellular components.
  • Competition assays with different NLS peptides.
  • Binding studies with isolated nuclei.

Related Experiment Videos

Main Results:

  • Identified 70- and 59-kD yeast proteins that bind to NLS peptides from SV-40 T antigen, Xenopus nucleoplasmin, Ga14, and histone H2B.
  • These binding proteins are associated with nuclei and extractable with salt.
  • Peptide-albumin conjugate binding to isolated nuclei is saturable and salt-extractable.
  • Cross-competition among different NLS peptides suggests a common recognition mechanism.

Conclusions:

  • The 70- and 59-kD yeast proteins exhibit properties consistent with receptors for nuclear localization signals.
  • These findings provide insight into the molecular machinery responsible for nuclear import in yeast.